{"id":1073,"date":"2026-08-12T07:34:56","date_gmt":"2026-08-12T07:34:56","guid":{"rendered":"https:\/\/www.fzs.unsa.ba\/?p=1073"},"modified":"2026-10-16T07:39:47","modified_gmt":"2026-10-16T07:39:47","slug":"dizajn-i-optimizacija-detekcije-sars-cov-2-virusa-lamp-metodom-za-dijagnosticku-upotrebu","status":"publish","type":"post","link":"https:\/\/www.fzs.unsa.ba\/en\/dizajn-i-optimizacija-detekcije-sars-cov-2-virusa-lamp-metodom-za-dijagnosticku-upotrebu\/","title":{"rendered":"Dizajn i optimizacija detekcije Sars-CoV-2 virusa LAMP metodom za dijagnosti\u010dku upotrebu"},"content":{"rendered":"<p class=\"wp-block-paragraph\">Od po\u010detka pandemije tragalo se za adekvatnom metodom za detekciju SARS-CoV-2 virusa. Kvantitativni PCR predstavlja \u201ezlatni standard\u201c u detekciji SARS-CoV-2, me\u0111utim iziskuje skupu infrastrukturu, vi\u0161esatnu analizu zbog \u010dega brza obrada velikog broja uzoraka predstavlja izazov. Cijena Loop-mediated isothermal Amplification (LAMP) metode po testu je znatno ni\u017ea od ostalih dostupnih molekularnih testova. Stoga se mnogi nau\u010dnici okre\u0107u ka optimizaciji i modifikaciji RT-LAMP metodologije kako bi jednakom senzitivno\u0161\u0107u odgovorili zahtjevima u dijagnostici COVID-19 bolesti. Pogotovo je ovo zna\u010dajno za laboratorije sa ograni\u010denim resursima u zemljama u razvoju, gdje nekontrolisano i neotkriveno \u0161irenje COVID-19 mo\u017ee rezultirati nepredvidivim ishodom. RT-LAMP metoda se zasniva na vrlo jednostavnom protokolu koji podrazumijeva izotermalnu amplifikaciju nukleinskih kiselina uz posebno dizajnirane prajmere. Detekcija rezultata se mo\u017ee vr\u0161iti na vi\u0161e na\u010dina, a u ovom istra\u017eivanju \u0107e se koristiti spektrofotometrija kao metoda izbora. Za razliku od trenutno dostupnih molekularnih metoda za detekciju SARS-CoV-2, ova metoda je veoma prakti\u010dna, brza i instrumentalno neovisna. Kanton Sarajevo se do sada susretao sa tri vala pandemije, koja su se te\u0161ko stavljala pod kontrolu zbog nemogu\u0107nosti pravovremenog testiranja simptomatskih, asimptomatskih pacijenata, kao i osoba koje su bile u kontaktu sa zara\u017eenim pacijentima. Pomo\u0107u ove metode, testiranje \u0107e biti znatno dostupnije gra\u0111anima, a rezultati \u0107e se dobijati mnogo br\u017ee sa jednakom ta\u010dno\u0161\u0107u. <\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>Klju\u010dne rije\u010di: LAMP, SARS-CoV-2, Real-time PCR, spektrofotomerija, RNK, reverzna transkripcija, prajmeri<\/strong><\/p>\n\n\n<style>.wp-block-kadence-advancedbtn.kb-btns1073_ff147b-b7{gap:var(--global-kb-gap-xs, 0.5rem );justify-content:center;align-items:center;}.kt-btns1073_ff147b-b7 .kt-button{font-weight:normal;font-style:normal;}.kt-btns1073_ff147b-b7 .kt-btn-wrap-0{margin-right:5px;}.wp-block-kadence-advancedbtn.kt-btns1073_ff147b-b7 .kt-btn-wrap-0 .kt-button{color:#555555;border-color:#555555;}.wp-block-kadence-advancedbtn.kt-btns1073_ff147b-b7 .kt-btn-wrap-0 .kt-button:hover, .wp-block-kadence-advancedbtn.kt-btns1073_ff147b-b7 .kt-btn-wrap-0 .kt-button:focus{color:#ffffff;border-color:#444444;}.wp-block-kadence-advancedbtn.kt-btns1073_ff147b-b7 .kt-btn-wrap-0 .kt-button::before{display:none;}.wp-block-kadence-advancedbtn.kt-btns1073_ff147b-b7 .kt-btn-wrap-0 .kt-button:hover, .wp-block-kadence-advancedbtn.kt-btns1073_ff147b-b7 .kt-btn-wrap-0 .kt-button:focus{background:#444444;}<\/style>\n<div class=\"wp-block-kadence-advancedbtn kb-buttons-wrap kb-btns1073_ff147b-b7\"><style>ul.menu .wp-block-kadence-advancedbtn .kb-btn1073_620a2c-a7.kb-button{width:initial;}.wp-block-kadence-advancedbtn .kb-btn1073_620a2c-a7.kb-button{padding:var(--kb-btn-padding);margin:var(--kb-btn-margin);box-shadow:none;}.wp-block-kadence-advancedbtn .kb-btn1073_620a2c-a7.kb-button:hover, .wp-block-kadence-advancedbtn .kb-btn1073_620a2c-a7.kb-button:focus{box-shadow:var(--kb-btn-shadow-hover, none);}<\/style><a class=\"kb-button kt-button button kb-btn1073_620a2c-a7 kt-btn-size-standard kt-btn-width-type-auto kb-btn-global-fill kt-btn-has-text-true kt-btn-has-svg-false wp-block-kadence-singlebtn\" href=\"https:\/\/www.fzs.unsa.ba\/wp-content\/uploads\/2026\/10\/Dizajn-i-optimizacija-detekcije-Sars-CoV-2-virusa-LAMP-metodom-za-dijagnosticku-upotrebu.pdf\"><span class=\"kt-btn-inner-text\">Download PDF<\/span><\/a><\/div>","protected":false},"excerpt":{"rendered":"<p>Od po\u010detka pandemije tragalo se za adekvatnom metodom za detekciju SARS-CoV-2 virusa. Kvantitativni PCR predstavlja \u201ezlatni standard\u201c u detekciji SARS-CoV-2,&#8230;<\/p>","protected":false},"author":1,"featured_media":1064,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"_kad_post_classname":"","footnotes":""},"categories":[17],"tags":[],"class_list":["post-1073","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-projekti"],"_links":{"self":[{"href":"https:\/\/www.fzs.unsa.ba\/en\/wp-json\/wp\/v2\/posts\/1073","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.fzs.unsa.ba\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.fzs.unsa.ba\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.fzs.unsa.ba\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.fzs.unsa.ba\/en\/wp-json\/wp\/v2\/comments?post=1073"}],"version-history":[{"count":1,"href":"https:\/\/www.fzs.unsa.ba\/en\/wp-json\/wp\/v2\/posts\/1073\/revisions"}],"predecessor-version":[{"id":1088,"href":"https:\/\/www.fzs.unsa.ba\/en\/wp-json\/wp\/v2\/posts\/1073\/revisions\/1088"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.fzs.unsa.ba\/en\/wp-json\/wp\/v2\/media\/1064"}],"wp:attachment":[{"href":"https:\/\/www.fzs.unsa.ba\/en\/wp-json\/wp\/v2\/media?parent=1073"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.fzs.unsa.ba\/en\/wp-json\/wp\/v2\/categories?post=1073"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.fzs.unsa.ba\/en\/wp-json\/wp\/v2\/tags?post=1073"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}